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Detection of intranuclear clusters of Sm antigens with monoclonal anti‐Sm antibodies by immunoelectron microscopy
Author(s) -
Eliceiri George L.,
Ryerse Jan S.
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041210226
Subject(s) - snrnp , immunoelectron microscopy , small nuclear ribonucleoprotein , ribonucleoprotein , monoclonal antibody , microbiology and biotechnology , small nuclear rna , immunostaining , biology , virology , antibody , cell nucleus , nucleus , rna , cajal body , chemistry , rna splicing , immunohistochemistry , biochemistry , genetics , immunology , non coding rna , gene
It has been shown that small nuclear RNA (snRNA) species U1, U2, U4, U5, and U6 are found in the nucleus in the form of small nuclear ribonucleoprotein particles (snRNPs), and that anti‐Sm antibodies react with snRNP polypeptides, which are associated with all five snRNAs. We report here a novel intranuclear complex, denoted “Sm cluster,” detected by immunostaining with monoclonal anti‐Sm antibodies in HeLa cells.