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Treatment of lymphoblastoid cells with interferon decreases insulin binding
Author(s) -
Faltynek Connie R.,
McCandless Sarah,
Baglioni Corrado
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041210224
Subject(s) - transferrin , insulin , lymphoblast , interferon , medicine , endocrinology , growth inhibition , transferrin receptor , receptor , cell culture , hep g2 , raji cell , chemistry , biology , cell growth , in vitro , biochemistry , immunology , genetics
Lymphoblastoid Daudi cells, which are highly sensitive to growth inhibition by interferon (IFN), can be grown in a defined serum‐free medium containing insulin, transferrin, and albumin as the only proteins. We examined whether the growth inhibition by IFN could be in part due to a change in receptors for insulin or transferrin. Cells treated for at least 2 days with 100 units/ml of IFN‐α2 bound less 125 I‐insulin and after 3 days of treatment this binding was reduced by more than 50%. No change in the binding of 125 I‐transferrin was observed. Treatment with IFN of Raji cells, which are resistant to growth inhibition by IFN, resulted in a similar decrease in 125 I‐insulin binding. Growth inhibition of Daudi cells by serum deprivation had no effect on 125 I‐insulin binding. Therefore, the IFN‐induced loss of insulin binding sites is not a consequence of growth inhibition.