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Control of DNA synthesis in polyploid mammalian cells
Author(s) -
Graves Jennifer A. Marshall,
McMillan Julie
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041210219
Subject(s) - polyploid , nuclear dna , dna synthesis , ploidy , dna , biology , nucleus , chinese hamster , microbiology and biotechnology , genetics , biophysics , gene , mitochondrial dna
Abstract To test the hypothesis that the duration of DNA synthesis is an inverse function of nuclear size or DNA content, the S phase was calculated from PLM analysis for pseudodiploid, tetraploid, and octaploid lines of Chinese hamster cells growing as a monolayer or in suspension. S phase times were found not to be significantly different between polyploid lines and the diploid lines from which they were derived, regardless of the conformation of the nucleus. There is no evidence, therefore, that would implicate the nuclear membrane, or nuclear surface area/volume relationships, in the control of DNA synthesis.