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β‐Adrenergic stimulation inhibits calcium efflux and alters the morphology of cultured arterial muscle cells
Author(s) -
Smith Jeffrey Bingham
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041210215
Subject(s) - stimulation , intracellular , efflux , colchicine , microbiology and biotechnology , calcium , antagonist , receptor , endocrinology , medicine , cell , chemistry , biology , biochemistry
β‐Adrenoreceptor stimulation with isoproterenol (IP) rapidly and reversibly rounded and arborized smooth muscle cells (SMC) cultured from rat aorta. The arborized SMC remained firmly attached to the substratum via a network of long, dendritelike processes. Arborization of the SMC correlated closely with increases in cellular cAMP produced by IP and a variety of other compounds. The intracellular calcium antagonist 8‐(N,N‐diethylamino)‐octyl‐3,4,5‐trimethoxybenzoate (TMB‐8) also rounded and arborized the SMC. Antitubulin compounds potently blocked arborization by IP, dibutyryl cAMP, and TMB‐8. The release of cell‐bound 45 Ca 2+ was followed in the presence and absence of IP. IP strikingly increased the amount of 45 Ca 2+ that remained cell bound between 20 and 120 min Propranolol and colchicine prevented IP from inhibiting the release of cell‐bound 45 Ca 2+ . These results suggest that the modulation of Ca 2+ transport is involved in the arborization of cultured SMC by cAMP.