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Inhibition of cell communication between Balb/c 3t3 cells by tumor promoters and protection by cAMP
Author(s) -
Enomoto Taira,
Martel Nicole,
Kanno Yoshinobu,
Yamasaki Hiroshi
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041210209
Subject(s) - cycloheximide , 3t3 cells , chinese hamster , intracellular , cell culture , microbiology and biotechnology , hamster , phorbol , cell , biology , downregulation and upregulation , phorbol ester , tetradecanoylphorbol acetate , protein biosynthesis , chemistry , biochemistry , protein kinase c , signal transduction , transfection , genetics , gene
The effect of phorbol ester tumor promoters on the communication between individual cells in confluent culture was studied using a fluorescent dye transfer method. Cell‐cell communication between mouse Balb/c 3T3 cells and between Chinese hamser V79 cells was inhibited almost completely by tumor‐promoting phorbol esters, but not by nonpromoting derivatives; the effect was reversed upon removal of the promoter. Intercellular communication between Balb/c 3T3 cells, but not Chinese hamster V79 cells, was increased significantly in the presence of dbcAMP and caffeine, and these compounds counteracted the effects of tumor promoters. Inhibition of cell communication by phorbol esters appears to be receptor‐mediated, since specific binding of 3 H‐phorbol‐12,13‐dibutyrate to Balb/c 3T3 cells was inhibited only by compounds that also inhibit intercellular dye transfer. A study with cycloheximide suggests that the reversible inhibition of intercellular communication by phorbol esters may not need de novo protein synthesis, while upregulation of communication by cAMP requires protein synthesis.