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The role of iron in the growth of human leukemic cell lines
Author(s) -
Titeux Monique,
Testa Ugo,
Louache Fawzia,
Thomopoulos Pierre,
Rochant Henri,
BretonGorius Jeanine
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041210131
Subject(s) - transferrin , hemin , ferric , cell culture , hemoglobin , transferrin receptor , chemistry , biochemistry , ammonium sulfate , cell growth , biology , heme , chromatography , inorganic chemistry , genetics , enzyme
The growth requirements of three human leukemic cell lines (K 562, HEL, U937) have been studied in the absence of serum. For growth in serum‐free medium, the cells require insulin, transferrin, and albumin. Two highly watersoluble iron salts, ferric ammonium citrate and ferric ammonium sulfate, may completely replace transferrin for supporting the growth of these cell lines. Similar results were obtained when mitogen‐stimulated lymphocytes were grown in serum‐free media. Iron containing compounds, such as hemin or hemoglobin, were also able to replace transferrin. Experiments using 42/6 monoclonal antibody strongly suggest that free‐iron salts are taken up by the cells by a mechanism that is completely independent from transferrin receptors.