Premium
Characterization of monovalent ion transport systems in an insect cell line ( Manduca sexta embryonic cell line che)
Author(s) -
English Leigh H.,
Cantley Lewis C.
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041210115
Subject(s) - dids , vanadate , extracellular , cotransporter , manduca sexta , ion transporter , furosemide , biophysics , intracellular , chemistry , sodium , cell culture , biochemistry , biology , insect , botany , membrane , genetics , organic chemistry
The monovalent ion transport systems of an immortalized insect cell line (CHE) have been investigated. These cells are unusual in that unlike most vertebrate cells, their normal extracellular environment consists of high potassium and low sodium concentrations. CHE cells maintained high intracellular [K + ] through both a furosemide‐inhibitable and a vanadate‐inhibitable transport system. Intracellular exchangeable [Na + ] was slightly lower than the extracellular [Na + ] and was maintained at this level through a vanadate‐sensitive transport system. Na + uptake was also inhibited by furosemide: however, the stoichiometry of furosemide‐sensitive Na + uptake when compared with furosemide‐sensitive K + uptake indicated that these cations are not cotransported. 4,4′‐Diisothiocyano‐2,2′‐disulfonic acid stilbene (DIDS) inhibited Na + , K + , and Cl − uptake. Vanadate and furosemide decreased cytoplasmimic pH, while cytoplasmic pH increased in the presence of DIDS. A model is presented explaining how Na + , K + , Cl − , H + and HCO 3 − fluxes are regulated in these cells.