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Effects of sodium butyrate on growth and cell‐cycle kinetics of cultured rabbit articular chondrocytes
Author(s) -
Larno Suzanne,
Ronot Xavier,
Adolphe Monique,
Lechat Paul
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041200317
Subject(s) - sodium butyrate , rabbit (cipher) , kinetics , chemistry , microbiology and biotechnology , cell growth , cell cycle , sodium , cell , biophysics , biology , biochemistry , gene , statistics , physics , mathematics , organic chemistry , quantum mechanics
The sodium salt of n‐butyric acid was found to inhibit the growth of asynchronous cultures of rabbit articular chondrocytes. This inhibitory effect was dose‐dependent between 1 mM and 5 mM, reversible, and accompanied by volume enhancement and modification of cellular morphology. Flow‐cytometric analysis showed that drug exposure led to a slowing‐down of the cell‐cycle progression; after 1 day's exposure, cells accumulated in G1, and after 2 or 3 days' treatment, in G2, without a blockage in M; the increase of cells in G2 was in fact due to an enhancement of binculeated cells. The treated cells had an increased RNA content. Articular chondrocytes seem to be target cells for sodium butyrate and therefore it represents a valuable biological tool for studying the mechanisms of their growth regulation.