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Prostaglandin synthesis by cells comprising the calf pulmonary artery
Author(s) -
Menconi Michael,
Hahn Gerald,
Polgar Peter
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041200209
Subject(s) - bradykinin , prostacyclin , medicine , prostaglandin , endocrinology , fibroblast , cell culture , endothelial stem cell , angiotensin ii , prostaglandin e2 , cell type , thromboxane a2 , endothelium , biology , stimulation , vascular smooth muscle , cell , chemistry , microbiology and biotechnology , smooth muscle , in vitro , biochemistry , receptor , genetics , blood pressure
Prostaglandin (PG) production was evaluated in the three cell types (endothelial, smooth muscle, and fibroblast) comprising the bovine pulmonary artery. Prostacyclin (PG1 2 ) was the predominant prostaglandin (PG) produced by endothelial, smooth muscle, and fibroblast cells as they exist in culture or in freshly excised tissue fragments. In addition to PGl 2 , measurable amounts of PGE 2 , PGF 2a , and thromboxane A 2 (TXA 2 ) were also produced by these cells. Endothelial cells were the most active producers of PGs. However, the type of PG produced was characteristic of the particular cell type, while the level of production was dependent on external factors. Prostaglandin production by cultured cells, both under basal conditions and in response to stimulatory agents, was quite similar to that of the respective freshly excised tissue fragments containing a given cell type. These cells in culture could be stimulated to produce PGl 2 by both angiotensin and bradykinin at very low (physiological) concentrations, a further indication of the retention of the physiological responsiveness of these cells in culture. Endothelial cells and fibroblasts were activated by bradykinin at concentrations as low as 10 −12 M but did not respond to angiotensin. Smooth muscle cells in primary and first passage cultures were activated by both bradykinin and angiotensin at 10 −12 M concentrations. Serial subcultivations of smooth muscle cells resulted in a progressive loss in their responsiveness to bradykinin stimulation. The state of cell growth proved to be an important determinant of PG production. Actively growing cells in culture synthesized less PG when compared to cells which had entered into a “quiescent” nongrowth state.