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Hydrocortisone stimulates fibronectin synthesis in cultured fibroblasts
Author(s) -
Lien YeongHau,
Wong Mitchell J.,
Golbus Mitchell S.,
Stern Robert
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041200114
Subject(s) - fibronectin , foreskin , hydrocortisone , fibroblast , microbiology and biotechnology , cell culture , gel electrophoresis , polyacrylamide gel electrophoresis , chemistry , human skin , biology , cell , biochemistry , in vitro , genetics , enzyme
The effect of hydrocortisone on fibronectin synthesis was investigated in cultured skin fibroblasis. Confluent cells were treated with hydrocortisone (10 −7 M to 10 −5 M) for 2 days and labeled with [ 3 H]proline for 24 h. Fibronectin levels in both the culture medium and the cell layer were studied by gelatin‐Sepharose affinity chromatography and SDS‐polyacrylamide gel electrophoresis. In control cultures of human fetal skin fibroblasts, fibronectin constituted 8% of the total labeled proteins in the medium. The proportion of fibronectin increased to 13.1% at 10 −7 M hydrocortisone, 15.5% at 10 −6 M and to 19.4% at 10 −5 M. The proportion of fibronectin associated with the cell layer remained at 2‐3% of total [ 3 H]prolne‐labeled proteins and did not increase with hydrocortisone exposure. The stimulating effect of hydrocortisone on medium fibronectin was also demonstrated in cultured human newborn foreskin fibroblasts and in rabbit skin fibroblasts.