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Clonal generation of multipotent and unipotent hemopoietic blast cell colonies in vitro
Author(s) -
Keller Gordon,
Holmes Wendy,
Phillips Robert A.
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041200105
Subject(s) - biology , haematopoiesis , colony forming unit , spleen , in vitro , bone marrow , macrophage , immunology , microbiology and biotechnology , andrology , stem cell , genetics , bacteria , medicine
Colonies with a unique compact morphology and displaying high replating potential develop in methycellulose cultures when bone marrow cells from hydroxyurea‐ or 5‐fluorouracil‐treated mice are cultured in the presence of syngeneic thymocytes. These compact colonies are clonal, originating from a single unit, the compact‐colony‐forming unit (CFU). Based on replating analysis, at least three groups of compact colonies exist: (1) those that do not replate, (2) those that replate and give only GM colonies, and (3) those that give rise to granulocyte‐macrophage (GM), erythroid (E), and mixed‐erythroid (MIX/E) colonies. Colonies in the last group have a mean size of 2,060 cells and generate an average of 244 colonies in secondary cultures. Velocity sedimentation studies showed the compact‐CFU to be similar in size to the MIX/E‐CFU, sedimenting at rates between 3.5 and 7 mm/hour. In addition to giving rise to large numbers of in vitro CFU, compact‐CFU are also able to generate day 8 spleen colony‐forming units (CFU‐S).