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Insulin inhibits the glucocorticoid‐mediated increase in hepatocyte EGF binding
Author(s) -
Lin Qixiong,
Blaisdell Joyce,
O'Keefe Edward,
Earp H. Shelton
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041190304
Subject(s) - glucocorticoid , glucocorticoid receptor , endocrinology , medicine , epidermal growth factor , insulin , dexamethasone , basal (medicine) , hepatocyte , hydrocortisone , receptor , hormone , biology , chemistry , in vitro , biochemistry
Hydrocortisone and dexamethasone produced a time‐dependent increase [ 125 I]epidermal growth factor ([ 125 I]EGF) binding in primary cultures of isolated rat hepatocytes. Maximally effective doses of glucocorticoids resulted in a 70–100% increase in binding. The effect was similar when hepatocytes were maintained on collagen‐coated plates or directly on culture dishes. The glucocorticoid‐mediated increase in [ 125 I]EGF binding could be detected after 4 h exposure to glucocorticoid and was substantial by 8 h. The major effect of glucocorticoid appeared to be to increase the number of EGF receptors. While insulin (100 nM) had no effect on basal [ 125 I]EGF binding, it significantly inhibited the increase produced by the glucocorticoid. Since the inhibitory effect of insulin was only observed when insulin was added with the inducing glucocorticoid, insulin appears to inhibit an early hydrocortisone‐mediated event.