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Development of calcium and secretory responses in the human promyelocytic leukemia cell line HL60
Author(s) -
Naccache P. H.,
Molski T. F. P.,
Spinelli B.,
Borgeat P.,
Abboud C. N.
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041190215
Subject(s) - hl60 , microbiology and biotechnology , cellular differentiation , cytochalasin b , receptor , biology , chemotaxis , leukotriene b4 , calcium , chemistry , leukemia , immunology , cell , biochemistry , inflammation , organic chemistry , gene
We have begun to characterize the development of the excitation – response coupling sequence in the human promyelocytic leukemia cell line HL60. Using the recently developed fluorescent calcium probe quin‐2, it was found that DMSO induced myeloid differentiation of the HL60 cells is accompanied by the development of a calcium response to the addition of the chemotactic factors fMet‐Leu‐Phe and leukotriene B 4 . The characteristics (time course, concentration dependence, stereospecificity, and metabolic dependence) of the calcium response are extremely similar to those previously described in human neutrophils. These results imply that functional receptors for leukotriene B 4 appear in HL60 cells upon the induction of differentiation and also lend strong support to the use of these HL60 cells as a model of human myeloid differentiation. We have also characterized the emergence of a secretory response to fMet‐Leu‐Phe and leukotriene B 4 in cytochalasin B treated HL60 cells. In addition, it is found that differentiation was required for the calcium ionophore A23187 to express its secretory activity toward the HL60 cells. This last set of results implies that differentiation is accompanied by the coordinated appearance of surface receptors and cytoplasmic factors required for the expression of cellular responsiveness.