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Chinese hamster ovary cell variants resistant to monensin, an ionophoric antibiotic. II. Growth requirement for insulin and altered insulin‐receptor activity
Author(s) -
Sato Yasufumi,
Ono Mayumi,
Takaki Ryosaburo,
Kuwano Michihiko
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041190210
Subject(s) - chinese hamster ovary cell , monensin , insulin , endocrinology , medicine , insulin receptor , biology , receptor , ovary , chemistry , insulin resistance , biochemistry
From the Chinese hamster ovary (CHO) cell, genetic variants (Mon R –31 and Mon R –32) relatively resistant to monensin, an ionophoric antibiotic, have been isolated. Growth of both Mon R ‐31 and Mon R ‐32 clones required higher doses of serum than CHO. Addition of insulin to media containing a low dose of serum restored full colony formation, but growth of Mon R ‐31 or Mon R ‐32 cells required more insulin than CHO cells. Specific binding of [ 125 l]insulin was observed in these cell lines. The two Mon R clones bound about one‐half or less the [ 125 l]insulin bound by CHO cells. Scatchard analysis for [ 125 l]insulin binding at 4°C and 37°C showed altered number of binding sites, but not insulin affinity: The number of binding sites in the Mon R cell was about a half or less that of the parental CHO cell. Down‐regulation of insulin receptor was assayed when both CHO and Mon R cells were incubated with 1 μg/ml insulin. A 50–60% decrease in levels of insulin surface binding capacities was observed in CHC after exposure to insulin, whereas there was no decrease in Mon R cell. The cellular uptake of 2‐[ 3 H]deoxyglucose into CHO cells was significantly enhanced in the presence of insulin, but only slight, if any, increase was observed in Mon R cells.