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Bioassay of retinoids using cultured human conjunctival keratinocytes
Author(s) -
Gilfix Brian M.,
Green Howard
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041190205
Subject(s) - keratin , bioassay , retinoic acid , keratinocyte , retinoid , chemistry , biochemistry , in vitro , filaggrin , biology , microbiology and biotechnology , immunology , gene , genetics , atopic dermatitis
The biological action of retinoids has been assayed using the differentiated properties of cultured human conjunctival keratinocytes. The effects measured were the suppression of envelope cross‐linking and the promotion of synthesis of a keratin of molecular weight 40,000. Among the retinoids tested, the most powerful was the arotinoid Ro 13–6298, which reduced envelope formation detectably at 10 −11 M and by 90% at a concentration of 2 × 10 −10 M. The arotinoid was about 15 times more potent than trans‐retinoic acid. The order of effectiveness of the retinoids in suppressing envelope cross‐linking was the same as the order of effectiveness in promoting the synthesis of the 40‐kd keratin.