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Transaminase inhibitors block glycolysis and G 1 to S phase progression in chick embryo fibroblasts: Reversal by α‐keto acids
Author(s) -
Groelke John,
Amos Harold
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041190121
Subject(s) - transaminase , glycolysis , embryo , citrate synthase , insulin , biochemistry , biology , citric acid cycle , cycloserine , enzyme , chemistry , endocrinology , microbiology and biotechnology , antibiotics
Two transaminase inhibitors, aminooxyacetate and cycloserine, inhibited the initiation of insulin‐stimulated DNA synthesis in chick embryo fibroblasts. This inhibition was overcome when pyruvate (4 mM), oxaloacetate (4 mM), or α‐ketobutyrate (10 mM) was included in the culture medium with hormone and inhibitor. Aminooxyacetate also inhibited lactate production in insulin treated cultures in the absence of added α‐keto acid.