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Fibroblast spreading and phagocytosis: Similar cell responses to different‐sized substrata
Author(s) -
Grinnell Frederick
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041190110
Subject(s) - phagocytosis , fibronectin , cytochalasin b , receptor , cell , microbiology and biotechnology , biology , cytochalasin , cell culture , cell adhesion , cytochalasin d , wheat germ agglutinin , baby hamster kidney cell , adhesion , fibroblast , phagocytic cell , cell migration , chemistry , biophysics , biochemistry , cytoskeleton , lectin , genetics , organic chemistry
Experiments were carried out to test the hypothesis that cell spreading and phagocytosis are similar cell responses to different‐sized substrata. The following morphological and biochemical studies provided evidence for this supposition. Cells phagocytosed 1.09‐μm and 5.7‐μm latex beads, but were unable to completely ingest 15.8‐μm or 25.7‐μm beads. With the larger beads, the cells spread around the bead surfaces with an appearance typical of cells spread on culture dishes. Biochemical studies with cytochalasin D, azide, and iodoacetate, as well as temperature‐dependence studies, demonstrated similar responses of cell spreading and phagocytosis to these treatments. Similar cell surface receptors were involved in cell spreading and phagocytosis based upon experiments using antibodies to baby hamster kidney (BHK) cell wheat germ agglutinin receptors. And finally, BHK cell variants with defective plasma fibronectin (pFN) receptors were unable to spread on pFN‐coated dishes or ingest pFN‐coated beads. Evidence also is presented concerning the “contact” process in cell adhesion. It was found that azide and low temperature inhibited cell attachment per se but did not block fibronectin‐receptorinter actions based upon cell binding of pFN‐coated beads. A possible explanation for the contact process is presented based upon the resistance of cells and beads to shear forces.