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Prostaglandin F 2α stimulates phosphatidylinositol turnover and increases the cellular content of 1,2‐diacylglycerol in confluent resting swiss 3T3 cells
Author(s) -
Macphee Colin H.,
Drummond Alan H.,
Otto Angela M.,
de Asua Luis Jimenez
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041190107
Subject(s) - phosphatidylinositol , diacylglycerol kinase , dna synthesis , epidermal growth factor , alpha (finance) , endocrinology , prostaglandin , stimulation , second messenger system , prostaglandins f , medicine , biology , microbiology and biotechnology , chemistry , biochemistry , signal transduction , protein kinase c , dna , receptor , construct validity , nursing , patient satisfaction
Prostaglandin F 2α (PGF 2α ); which stimulates DNA synthesis in resting 3T3 cells, also stimulates the incorporation of [ 32 P]PO 4 into phosphatidylinositol. The effect is selective for PGF 2α when compared with PGE 1 , PGE 2 , and PGF 2α . Epidermal growth factor (EGF) also stimulates DNA synthesis but does not affect phosphatidylinositol turnover. PGE 1 , which acts synergistically with PGF 2α to enhance DNA synthesis, does not affect the ability of PGF 2α to enhance the incorporation of [ 32 P]PO 4 into phosphatidylinositol. PGF 2α also causes a small increase in the cellular content of 1,2‐diacylglycerol. This effect is not shared by EGF or PGE 1 . Stimulation of phosphatidylinositol metabolism resulting in an increase in the cellular content of 1,2‐diacylglycerol may thus constitute an event in the pathway leading to the initiation of DNA synthesis in which PGF 2α differs in its action from EGF.