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Coordinate regulation of glycolysis by hypoxia in mammalian cells
Author(s) -
Robin Eugene D.,
Murphy Brian J.,
Theodore James
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041180311
Subject(s) - glycolysis , enzyme , hypoxia (environmental) , microbiology and biotechnology , limiting , biochemistry , cytoplasm , adenosine triphosphate , biology , metabolism , chemistry , oxygen , mechanical engineering , organic chemistry , engineering
The impact of hypoxic exposure on the activities of all 11 glycolytic enzymes was studied in cell culture into mammalian cells—mouse lung macrophages and L8 rat skeletal muscle cells. During hypoxic exposure, the measured activity of all glycolytic enzymes increased, establishing coordinate regulation. Three nonglycolytic cytoplasmic enzymes showed no change in activity under the same conditions, suggesting a specific mechanism. Hypoxia appears to increase the activities of all glycolytic enzymes whether rate‐limiting or not, presumably increasing adenosine triphosphate availability despite decreased O 2 supply.