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In vitro demonstration of deleterious effect of steel mutation on spermatogenesis in mice
Author(s) -
Nishimune Yoshitake,
Haneji Tatsuji,
Maekawa Mamiko,
Kitamura Yukihiko
Publication year - 1984
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041180105
Subject(s) - spermatogenesis , biology , in vitro , sertoli cell , mutant , mutation , germ cell , medicine , andrology , mitosis , endocrinology , germ , microbiology and biotechnology , genetics , gene
The effect of the steel mutation on spermatogenesis was investigated by using organ culture. Cultured cryptorchid testes from C57BL/6− +/+ mice showed an effective differentiation of type A spermatogonia in response to an increased concentration of bovine serum. In contrast, the cryptorchid testes from C57BL/6 – S / d / + mice showed only limited differentiation of type A spermatogonia even in the highest concentration of serum tested. The type A spermatogonia of mutant mice showed the normal mitotic response but did not differentiate further. The findings reported here are the first in vitro demonstration of the deleterious effects of the steel mutation on testicular germ cell differentiation and suggest that the effect of the steel mutation is expressed in Sertoli cells, the only supporting element in seminiferous tubules, or germ cells themselves.