Concanavalin A increases spontaneous beat rate of embryonic chick heart cell aggregates

The plant lectin concanavalin A (Con A), at concentrations of 5–200 μg/ml, induced a twofold to fivefold increase in spontaneous beat rate of cultured aggregates of ventricular cells from seven‐day chick embryos. This response was time, dose, and temperature dependent and was accompanied by a decrease in transmembrane potential. It could be blocked or reversed by α‐methyl‐D‐mannoside but was not reversed by dilution alone. Binding of the lectin occurred in the cold, but a temperature‐dependent process was also necessary to produce the response. Divalent (succinyl) Con A did not cause a beat rate increase. Whole heart aggregates responded similarly but less intensely than ventricular aggregates. Atrial aggregates, and whole heart aggregates treated with 5 μg/ml of Con A, produced a biphasic chronotropic response, first decreasing then increasing their beat rates. These results suggest that saccharide‐bearing macromolecules on the heart cell surface play a role in regulating spontaneous beat rate.