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Growth effect of lithium on mouse mammary epithelial cells in serum‐free collagen gel culture
Author(s) -
Tomooka Yasuhiro,
Imagawa Walter,
Nandi Satyabrata,
Bern Howard A.
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041170303
Subject(s) - transferrin , epidermal growth factor , bovine serum albumin , cholera toxin , endocrinology , medicine , growth factor , fetal bovine serum , chemistry , lithium chloride , cell culture , insulin like growth factor , biology , biochemistry , cell , receptor , organic chemistry , genetics
Abstract The effect of lithium on the growth of mammary epithelial cells from adult virgin and midpregnant BALB/c or BALB/cfC3H mice was tested in a serum‐free collagen gel culture system. The serum‐free medium consisted of a 1:1 mixture of Ham's F12 and Dulbecco's Modified Eagle's medium supplemented with insulin, transferrin, cholera toxin, epidermal growth factor (EGF), and bovine serum albumin fraction V (BSA V). A multifold increase in cell number occurred during 10–12 days of culture in this medium. In dose‐response studies in which the concentration of each component of this serum‐free medium was varied in turn, the addition of LiCL (10 mM) enhanced growth at most concentrations of each factor. However, LiCL could not enhance growth in the absence of insulin or BSA V, but could replace EGF. The optimal concentration of LiCl was 5–10 mM; higher concentrations (20–80 mM) were toxic. KCl (1–10 mM) when added to the serum‐free medium slightly stimulated growth; the addition of NaCl to the medium had little effect on growth. LiCl did not enhance the growth of cells from spontaneous mammary tumors of BALB/cfC3H mice.