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Immunocytochemical localization of chick DNA polymerases α and β +
Author(s) -
Matsukage Akio,
Yamamoto Susumu,
Yamaguchi Masamitsu,
Kusakabe Moriaki,
Takahashi Taijo
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041170219
Subject(s) - dna polymerase , microbiology and biotechnology , biology , polymerase , dna polymerase i , proliferating cell nuclear antigen , dna clamp , dna , chromatin , mitosis , dna synthesis , dna replication , dna polymerase ii , reverse transcriptase , polymerase chain reaction , biochemistry , gene
An immunofluorescent method using specific antibodies was employed to detect DNA polymerases α and β in chick cells. With monoclonal antibodies produced by four independent hybridoma clones, most of the DNA polymerase α was shown to be present in nuclei of cultured chick embryonic cells. With a polyclonal, but highly specific, antibody against DNA polymerase β, this enzyme was also shown to be present in nuclei. DNA polymerase α was detected in proliferating cells before cell contact and in lesser amount in resting cells after cell contact, indicating that its content is closely correlated with cell proliferation. On the other hand, similar amounts of DNA polymerase β were detected in proliferating and resting cells. Furthermore, DNA polymerase β was detected in nuclei of most cells, while DNA polymerase α was detected only in large round nuclei in seminiferous tubules of chick testis. DNA polymerase α is presumably present in cells that are capable of DNA replication, and during the cell cycle it seems to remain in the nuclei during the G 1 , S, and G 2 phases, but to leave from condensed chromatin for the cytoplasm during the mitotic phase.