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Optimal conditions for proliferation of bone marrow‐derived mouse macrophages in culture: The roles of CSF‐1, serum, Ca 2+ , and adherence
Author(s) -
Hume David A.,
Gordon Siamon
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041170209
Subject(s) - thymidine , macrophage , bone marrow , macrophage colony stimulating factor , cell culture , microbiology and biotechnology , colony stimulating factor , cell growth , immunology , biology , chemistry , biochemistry , in vitro , haematopoiesis , genetics , stem cell
A method is described for the analysis of [ 3 H]‐thymidine incorporation in microtitre cultures of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating factor (CSF‐1). [ 3 H]‐thymidine incorporation depends on cell density, culture medium, and the concentration of CSF‐1 and serum, but is independent of Ca 2+ . Bone marrow‐derived macrophages are strongly adherent, but adherence can be dissociated from [ 3 H]‐thymidine incorporation.