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Properties of a cell growth inhibitor produced by mouse embryo fibroblasts
Author(s) -
Wells Valerie,
Mallucci Livio
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041170203
Subject(s) - thermolabile , sephadex , embryo , fractionation , methionine , microbiology and biotechnology , cell growth , polyacrylamide gel electrophoresis , biology , inhibitory postsynaptic potential , cell culture , chemistry , gel electrophoresis , biochemistry , chromatography , amino acid , enzyme , endocrinology , genetics
Secondary mouse embryo fibroblasts produce a growth inhibitor with the character of a thermolabile, nondialysable protein. The inhibitor was harvested from conditioned medium, and following G‐75 Sephadex fractionation it was isolated in one peak which consisted of two fractions eluting at approximately two thirds of the bed volume of the column where approximately 80 percent of the original activity was recovered with an increase in specific activity of about tenfold. Polyacrylamide gradient gel electrophoresis of fractions from L‐[ 35 S] methionine‐labelled conditioned medium showed that the two fractions with growth inhibitory activity contained some 4–5 bands and shared the two major components. Cell cycle studies showed that the growth inhibitory effect was exerted after addition during early and late G1 and during S phase, and morphological studies showed that where growth was inhibited the morphological expression of the cells was altered.