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Change in water proton relaxation time during erythrocyte maturation
Author(s) -
Cameron I. L.,
Dung H. C.,
Hunter K. E.,
Hazlewood C. F.
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041160320
Subject(s) - chemistry , relaxation (psychology) , hemoglobin , proton , nuclear magnetic resonance , biophysics , nuclear magnetic resonance spectroscopy , analytical chemistry (journal) , crystallography , biochemistry , chromatography , medicine , biology , stereochemistry , physics , quantum mechanics
Erythrocyte populations from newborn and mature mice were characterized according to: size; ultrastructural features; water content; concentration of intraerythrocyte elements including Na, Cl, K, P, S, Mg, and Fe; and the spin‐lattice (T 1 ) and spin‐spin relaxation times of water protons as measured by nuclear magnetic resonance (NMR) spectroscopy. A significant increase in the T 2 time from 142 ± 3 msec to 184 ± 3 msec occurred during erythrocyte maturation. This change in T 2 time was correlated with a change from a polyribosome‐rich hemoglobin‐poor cell type to a polyribosome‐absent hemoglobin‐rich cell type. The change in T 2 time could also be correlated to a significantly higher K and P concentration in the mature erythrocytes. The change in T 2 time was not correlated to a change in cellular water content or to the concentration of any of the other elements measured by electron probe X‐ray microanalysis. If the NMR relaxation times of water molecules truly reflect their average motional freedom, then the findings suggest that greater water ordering interaction occurs in the ribosome containing immature erythrocyte.