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Comparative energy metabolism in cultured heart muscle and hela cells
Author(s) -
Stanisz Jolanta,
Wice Burton M.,
Kennell David E.
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041150316
Subject(s) - glutamine , hela , metabolism , biochemistry , biology , fatty acid , carbohydrate metabolism , beta oxidation , amino acid , chemistry , cell
The yields of energy from oxidation of fatty acids, glucose, and glutamine were compared in cultures of chick embryo heart muscle (heart) and HeLa cells. Aerobic energy production, as measured by oxygen utilization, was comparable in the two cell types. In media containing dialyzed sera, the rates of incorporation of fatty acids directly into lipids were similar in both cells and accounted for > 97% of fatty acid metabolism in HeLa cells. However, in heart cells only 45% ended in lipid, 42% in protein, and 13% was released as CO 2 ; the latter two products probably reflect the oxidation of fatty acids to acetyl‐coenzyme A (‐CoA) and its subsequent metabolism in the citrate cycle. Increased serum concentration in the medium did not affect fatty acid metabolism in HeLa cultures, but resulted in greater oxidation by heart cells (> 100 times that by HeLa cells). The metabolisms of both glucose and glutamine were similar in heart and HeLa cells with ⩾ 60% of glucose carbon ending as medium lactate and only 3–5% converted to acetyl‐CoA. About 25% of glutamine carbon ended as CO 2 and increased utilizations with increasing serum concentrations was accountable in both cells by increased lactate from glucose and glutamate from glutamine. CO 2 production (and energy) from glutamine was independent of glutamine concentration within a tenfold range of physiological concentrations. The yields of energy have been calculated. In 10% dialyzed calf serum, oxidation of glutamine carbon provided about half of the total energy in heart cells, glucose about 35–45%, with most coming from glycolysis; oxidation of fatty acid carbon provided only 5–10%. That > 90% of the aerobic energy comes from glutamine in both cells can account for the comparable rates of oxygen utilization. HeLa cells derived little or no energy from fatty acids.