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Induction of reverse transformation and normal cell cycle regulation by dibutyryl cAMP in a chemically transformed cell line
Author(s) -
Wang Yong Chao,
Rao Potu N.
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041150307
Subject(s) - trypsinization , cell cycle , mitosis , dna synthesis , microbiology and biotechnology , premature chromosome condensation , biology , cell culture , theophylline , transformation (genetics) , bucladesine , cell , chemistry , dna , genetics , biochemistry , endocrinology , enzyme , gene , trypsin
The objective of this study was to determine whether N 6 , O 2 ‐dibutyryl 3′,5′‐adenosine monophosphate (db‐cAMP)‐induced reverse transformation in a chemically transformed mouse cell line, AKR‐MCA, would restore normal cell cycle regulation, particularly with regard to their growth arrest in the early G 1 period. The AKR‐MCA cells were grown to confluency in the presence or absence of db‐cAMP (0.5 mM) plus theophylline (1 mM). The confluent cultures were trypsinized and a portion of the cells were fused with mitotic HeLa cells to induce premature chromosome condensation, while the remaining cells were used to study the kinetics of initiation of DNA synthesis. The prematurely condensed chromosomes (PCC) of the control and the treated cultures were classified into G 1 , S, or G 2 types on the basis of their morphology. The G 1 PCC were further subclassified into six groups (+ 1‐ +6); +1 being the most condensed and +6 the most decondensed. The cyclic AMP (cAMP)‐treated cells exnibited better attachment to the culture dish, were blocked in early G 1 period at confluency, and entered S phase about 4 h later than the control following subculturing. In contrast, a majority of cells in the control cultures were arrested in S phase at confluency. These data indicate that the db‐cAMP‐induced reverse transformation in AKR‐MCA cells at least partially restores normal cell cycle regulation in these chemically transformed cells.

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