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Serum‐free culture of PC13 murine embryonal carcinoma cells
Author(s) -
Heath John K.,
Deller M. Jane
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041150302
Subject(s) - transferrin , endocrinology , medicine , cell culture , biology , carcinoma , carcinoma cell , cell growth , chemistry , microbiology and biotechnology , cell , andrology , biochemistry , genetics
The requirements for the serum‐free culture of PC13 murine embryonal carcinoma cells were determined. Supplementation of a 50:50 mixture of Dulbecco's modified Eagles medium and MCDB104 with transferrin (5 μg/ml), human high‐density lipoprotein (HDL) (100 μg/ml), and human low‐density lipoprotein (LDL) (50 μg/ml) supported growth comparable to that observed with 5% foetal calf serum. Media supplementation with lipoproteins apparently substitutes for the effects of insulin, desoctapeptide insulin (DOP), or multiplication‐stimulating activity (MSA) on EC cell multiplication. Clonal growth of PC13 EC cells in this serum‐free medium could only be achieved in the presence of suitable feeder cell monolayers. These observations demonstrate that PC13 EC cells do not have an absolute requirement for exogenous mitogens to support multiplication.