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Cell hybrids between SV40‐transformed macrophage cell lines and a Chinese hamster cell line: Growth responsiveness and induction of colony‐stimulating factor
Author(s) -
Ohki Kazunori,
Nagayama Ariki
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041140307
Subject(s) - biology , cell culture , macrophage , hamster , microbiology and biotechnology , cell growth , cell fusion , in vitro , chinese hamster ovary cell , biochemistry , genetics
Three cell lines from resident macrophages of BALB/c mice and four from activated macrophages of the same strain were isolated by infection with simian virus 40 (SV40). A majority of these cells showed dependency on L cell‐conditioned medium (LCM), which is necessary for proliferation of normal macrophages in vitro. Somatic cell hybridization was applied in the study of macrophage growth responsiveness. A macrophage cell line (BR 15) with strict dependency on LCM for growth was fused to a Chinese hamster cell line (hs222‐16); it was found that dependency on LCM was a dominant trait in the hybrids. Following fusion fo a macrophage cell line (BAM3) which grew without LCM to hs222‐16, a large number of colonies appeared in the selection medium containing LCM . Four hybrids not requiring LCM for growth were selected in an LCM‐free culture, and their hybrid properties were examined. Three out of the four hybrids secreted colony‐stimulating factor (CSF) constitutively, whereas the fourth secreted no CSF. The level of acid phosphatase activity in the hybrids was higher than in the parent cells. Two peaks of CSF activity were observed after gel filtration chromatography of conditioned medium: One was eluted at molecular weight of 36,000 and the other at 17,000.