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Glycerol‐3‐phosphate dehydrogenase is induced by glucocorticoids in hepatocytes and hepatoma cells in vitro
Author(s) -
Meyer Robert D.,
Preston Sheryl L.,
McMorris F. Arthur
Publication year - 1983
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041140209
Subject(s) - glucocorticoid , in vitro , cell culture , cytosol , dehydrogenase , biology , enzyme , endocrinology , medicine , dexamethasone , hepatocyte , enzyme inducer , microbiology and biotechnology , biochemistry , genetics
Previous studies have shown that cytosolic glycerol‐3‐phosphate dehydrogenase (GPDH; EC 1.1.1.8) can be induced by glucocorticoids in mammalian brain, mammary gland, and thymus, but it was thought that no induction occurred in liver. We report here that GPDH is induced by glucocorticoids in several lines of hepatoma cells and in rat hepatocytes cultured in vitro. When rat hepatoma cells of clone FU5AH were exposed to 3 μM hydrocortisone (HC) for 3 days, GPDH specific activity increased greater than sixfold over control. The rate and extent of induction were similar in exponentially growing and stationary‐phase cultures of cells. Four other hepatoma cell lines were inducible to a lesser extent, and three lines were not inducible. GPDH was also induced by glucocorticoids in cultures of hepatocytes isolated from livers of 6‐day‐old rats. The enzyme was induced threeto fourfold by the synthetic glucocorticoid, dexamethasone, in the presence of 1 nM insulin, but the induction was not observed in the absence of insulin.