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G‐CSF: Its relationship to leukemia differentiation‐inducing activity and other hemopoietic regulators
Author(s) -
Moore Malcolm A. S.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041130411
Subject(s) - haematopoiesis , leukemia , cellular differentiation , leukemia inhibitory factor , biology , cell culture , granulocyte , myeloid , granulocyte colony stimulating factor , myeloid leukemia , stimulation , immunology , cancer research , microbiology and biotechnology , stem cell , endocrinology , cytokine , biochemistry , genetics , chemotherapy , interleukin 6 , gene
The murine myelomonocytic leukemia WEHI‐3B exists as differentiation‐inducible (D + ) and noninducible (D − ) cell lines. Both lines produce a CSF species that stimulates exclusively the formation of neutrophil granulocyte colonies. This G‐CSF copurifies with a mast cell growth factor but can be separated from M‐ and GM‐CSF. NZB bone marrow is unresponsive to G‐CSF stimulation. WEHI‐3B D + cells can be induced to terminal granulocyte differentiation by a factor present in murine and human postendotoxin serum that is different from G‐CSF present in WEHI‐3B D + or D − CM since the latter has little or no leukemia differentiation‐inducing activity. Endotoxin treatment of C. parvum primed mice leads to simultaneous induction of serum activities with selective action on myeloid leukemic cells, a serum differentiation inducing activity and a leukemic colony inhibitory activity. These factors act synergistically to block leukemic stem cell self‐renewal. The results suggest that a variety of inducible factors may have potent and selective antileukemic activity.