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Involvement of Na + and HCO 3 − in receptor‐mediated endocytosis of α 2 ‐macroglobulin, epidermal growth factor, and vesicular stomatitis virus
Author(s) -
Dickson Robert B.,
Schlegel Richard,
Willingham Mark C.,
Pastan Ira H.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041130302
Subject(s) - endocytosis , epidermal growth factor , vesicular stomatitis virus , receptor mediated endocytosis , horseradish peroxidase , microbiology and biotechnology , biology , pinocytosis , receptor , cell culture , chemistry , biochemistry , enzyme , genetics
α 2 ‐Macroglobulin (α 2 M), epidermal growth factor (EGF), and vesicular stomatitis virus (VSV) each enter cultured fibroblasts by receptor‐mediated endocytosis. The present study defines some basic ionic requirements in the cell culture medium which are necessary for the maximal rate of endocytosis of these three ligands. Na + and HCO 3 −were both necessary for maximal endocytosis of 125 I‐α 2 M, 125 I‐EGF, and 35 S‐VSV at 37°C. The ion specificities for both the anion and cation requirements were established. The binding of 125 I‐α 2 M to its cellular receptors at 4°C was unaffected by the absence of Na + and HCO 3 −in the culture medium. In addition, the absence of Na + and HCO 3 −in the culture medium did not reduce cellular uptake of horseradish peroxidase by fluid phase endocytosis. Na + and HCO 3 −may be general requirements in receptor‐mediated endocytosis.