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Complementary recessive 25‐hydroxycholesterol‐resistant somatic cell mutants – assay of 25‐hydroxycholesterol binding activity
Author(s) -
Sinensky M.,
Logel J.,
Torget R.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041130220
Subject(s) - complementation , mutant , protein fragment complementation assay , biochemistry , enzyme , chemistry , microbiology and biotechnology , biology , gene
Complementation analysis of recessive 25‐hydroxycholesterol‐resistant mutants of the CHO‐KI cell shows the existence of at least two complementation groups, one of which is missing a binding activity for 25‐hydroxycholesterol. Both complementation groups are shown to be refractory to inhibition of cellular HMG‐CoA reductase activity and in the inhibition of biosynthesis of this enzyme by 25‐hydroxycholesterol.