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The competitive ability of stem cells from mice with Hertwig's anemia
Author(s) -
Barker Jane E.,
McFarland Eleanor C.,
Bernstein Seldon E.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041130212
Subject(s) - stem cell , haematopoiesis , biology , hemoglobin , bone marrow , immunology , anemia , macrocytic anemia , induced pluripotent stem cell , microbiology and biotechnology , medicine , endocrinology , genetics , embryonic stem cell , gene , biochemistry , vitamin b12
Abstract Mice homozygous for the gene, an, have a macrocytic, normochromic anemia. In this report, attempts have been made to cure Hertwig's anemia (an/an) by injecting genetically normal (+/+) stem cells. The anemia of unirradiated an/an mice was alleviated but not completely cured by injection of as many as 3 × 10 7 +/+ bone marrow cells. Lethal irradiation of the an/an recipients was necessary before injections of 10 7 +/+ marrow cells were effective in normalizing the blood parameters. The inability to achieve normal blood values without first destroying the host's own stem cells suggested that the indigenous an/an cells compete effectively with injected +/+ cells. This hypothesis was tested by injecting varying numbers of stem cells from C57BL/6J‐ +/+ mice, together with stem cells from either WBB6F 1 ‐an/an or, as controls, from their WBB6F 1 ‐ +/+ littermates, into lethally irradiated hosts. The C57BL/6J and WBB6F 1 mice have electrophoretically distinguishable hemoglobins. The an/an cells are able to compete in the repopulation of the host hematopoietic tissue as shown by the presence of WBB6F 1 hemoglobin in the recipients. The cells from mice with Hertwig's anemia, however, do not compete as effectively as do the same number of cells from the +/+ littermates. These results indicate that the pluripotent hematopoietic stem cells of an/an mice are reduced in number, seeding capacity, or proliferative potential.