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Glucocorticoid influence on growth of vascular wall cells in culture
Author(s) -
Longenecker John P.,
Kilty Laura A.,
Johnson Lorin K.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041130203
Subject(s) - glucocorticoid , fibroblast growth factor , endocrinology , medicine , in vitro , cell culture , biology , vascular smooth muscle , cell growth , hormone , endothelial stem cell , fibroblast , cell , smooth muscle , biochemistry , genetics , receptor
Abstract Primary mass cultures and cloned strains of bovine aortic endothelial and smooth muscle cells were investigated with respect to their growth responses to glucocorticoid hormones. The growth of primary endothelial cells was not influenced by glucocorticoid treatment in the absence of fibroblast growth factor (FGF) but was inhibited by about 30% in the presence of FGF; with cloned endothelial cells, glucocorticoids were also growth inhibitory only in the presence of FGF. In contrast, smooth muscle cell growth was inhibited 30%–70% by glucocorticoid treatment in both primary cultures and in the cloned strains in the absence of FCF, and this inhibition was totally abolished by the addition of FGF for both cultures. The corticosteroid influences on cell growth were glucocorticoid specific, concentration dependent, and were observed to be independent of the serum concentration. The results indicate that glucocorticoid hormones have direct and pronounced growth inhibiting effects on aortic smooth muscle cells but only minimal effects on endothelial cells when these components of the vascular wall are analyzed under identical conditions in vitro.