Premium
Effect of exogenous gangliosides on human neural cell division
Author(s) -
IcardLiepkalns Christine,
Liepkalns Vis A.,
Yates Allan J.,
Rodriguez Zoe R.,
Stephens Ralph E.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041130128
Subject(s) - thymidine , cell division , cell growth , cell , cell culture , biology , fetal bovine serum , glioma , cancer cell , biochemistry , microbiology and biotechnology , chemistry , cancer , in vitro , cancer research , genetics
Human neural cells in exponential growth phase were transferred to a serum‐free medium and maintained for 72 hr without any detectable loss in viability. The two normal fetal cell lines (CH I and CH II ) showed a serum‐dependent cell proliferation, but the glioblastoma multiforme cells (12–18) were able to continue proliferating in this totally synthetic medium. The incorporation of [ 3 H] thymidine into the acid‐precipitable fraction of both normal and neoplastic human neural cells was assayed in the presence and the absence of exogenous gangliosides by a convenient new method. In serum‐free medium, gangliosides (50 μM) inhibited the thymidine incorporation into the normal fetal cells within 24 hr and, in serum containing medium, reduced their proliferation within 48 hr. No such effects were detectable in the glioma cells. The inhibition of thymidine incorporation in the normal cells was reversible upon removal of the gangliosides. These results indicate a role of gangliosides in the postmitotic phase of normal human neural cells resulting in the regulation of cell proliferation.