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Temporal behavior of abrin in the intoxication of chinese hamster cells (line CHO)
Author(s) -
Tsuzuki Junji,
Wu Henry C.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041130116
Subject(s) - internalization , chinese hamster ovary cell , incubation , chemistry , cytoplasm , hamster , incubation period , toxin , microbiology and biotechnology , extracellular , biochemistry , biology , receptor
Abrin, a potent cytotoxin, was utilized as a probe to elucidate the mechanism by which external proteins are delivered to the cytoplasm of mammalian cells. Abrin bound rapidly to the surface receptors of the Chinese hamster cells (line CHO) and appeared to be internalized immediately without any significant lag. The maximum level of abrin internalization was achieved within eight minutes, based on both biochemical and electron microscopic autoradiographic studies with [ 125 |] abrin. About 10% of the silver grains of internalized [ 125 |] abrin were associated with vesicular structures, irrespective of the incubation time. Inhibition of protein synthesis began 30 minutes postincubation, and this latent period was not dependent on extracellular toxin concentration. SDS‐polyacrylamide gel electrophoresis of the internalized [ 125 |] abrin indicated that internalized abrin molecules remained intact even after two hours of incubation.