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Stimulation of embryonic mouse limb bud mesenchymal cell growth by peptide growth factors
Author(s) -
Kaplowitz Paul B.,
D'ercole A. Joseph,
Underwood Louis E.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041120307
Subject(s) - somatomedin , fibroblast growth factor , epidermal growth factor , biology , cell growth , limb bud , growth factor , stimulation , mesenchymal stem cell , microbiology and biotechnology , embryo , cell , insulin like growth factor , cell culture , endocrinology , medicine , biochemistry , genetics , receptor
The possible role of peptide growth factors in mammalian intrauterine cell growth has been investigated using primary cultures of undifferentiated mesenchymal cells from 11‐day mouse embryo limb buds. When grown as monolayer cultures, proliferation is greatly favored by high cell densities. In medium containing 0.2% serum, purified epidermal growth factor (EGF), fibroblast growth factor (FGF), multiplication stimulating activity (MSA), insulin, and somatomedin‐C (Sm‐C) do not increase cell growth, but a 30–40,000 molecular weight component of mouse fetal liver conditioned medium is stimulatory. On the other hand, when limb bud cells are grown as high density or micromass cultures, a method which better approximates in vivo growth conditions, all of the purified growth factors tested stimulate cell growth significantly. These growth factors have additive effects when used in combination, the best stimulation being observed with liver medium (10% v/v), EGF (10 ng/ml), FGF (200 ng/ml), and either insulin (1 m̈g/ml) or Sm‐C (20 ng/ml). We conclude that the response of limb bud cells to growth stimulation is influenced by the manner in which the cells are cultured and that at least four different growth factors are required for optimal in vitro proliferation. One of these, the active component of liver medium, appears to be a previously uncharacterized growth factor.