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The use of fluorescent dyes to measure membrane potentials: A critique
Author(s) -
Johnstone R. M.,
Laris P. C.,
Eddy A. A.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041120221
Subject(s) - cyanine , fluorescence , membrane , biophysics , membrane potential , chemistry , electrophysiology , measure (data warehouse) , biology , biochemistry , optics , computer science , neuroscience , physics , database
Under controlled conditions, fluorescent cyanine dyes can be used to mesure membrane potentials of cell suspensions. Similar changes in membrane potential can be followed both with fluorescent dyes and electrophysiological probes in response to changes in the ion composition of the medium. Recent reports that attempt to abrogate the use of the cyanine dyes in measurements of the membrane potential are misleading.