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Fibronectin inhibits morphological changes in cultures of vascular smooth muscle cells
Author(s) -
Brennan Michael J.,
Millis Albert J. T.,
Fritz Katherine E.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041120219
Subject(s) - fibronectin , monolayer , cell culture , transition (genetics) , smooth muscle , microbiology and biotechnology , biology , tissue culture , vascular smooth muscle , primary culture , chemistry , in vitro , biochemistry , endocrinology , extracellular matrix , genetics , gene
In culture, vascular smooth muscle cells proliferate until they form a confluent sheet of cells. At that time the morphology of the culture becomes altered and the cells form multilayered regions that eventually develop into nodular aggregations. We now demonstrate that the transition from monolayer culture to nodular culture is influenced by the presence of components in conditioned media. The development of nodules is enhanced by conditioned medium made from nodular cultures but is either inhibited or unaffected by monolayer culture‐conditioned medium. Examination of the two types of conditioned media using NaDodSO 4 ‐polyacrylamide gels reveals many similarities and one major difference. Nodular‐conditioned medium contains a prominent 42 kilodalton polypeptide which is not present in monolayer‐conditioned medium. Further, we demonstrate that although both nodular and monolayer cultures produce fibronectin the transition to nodular culture does not occur in the presence of exogeneously added plasma fibronectin.