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Analysis of purified fetal liver hemopoietic progenitor cells in liquid culture
Author(s) -
Nicola Nicos A.,
Metcalf Donald
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041120215
Subject(s) - pokeweed mitogen , haematopoiesis , progenitor cell , biology , immunology , macrophage , cell culture , andrology , microbiology and biotechnology , stem cell , medicine , biochemistry , concanavalin a , in vitro , genetics
The action of different types of colony‐stimulating factors (CSFs) on purified fetal hemopoietic progenitor cells in liquid cultures was studied. Three types of CSF were examined–pregnant mouse uterus extract (PMUE), which contains macrophage CSF; mouse lung conditioned medium (MLCM), which contains granulocyte‐macrophage CSF; and pokeweed mitogen‐stimulated spleen cell‐conditioned medium (PWM‐SCM), which contains ganulocyte‐macrophage, eosinophil, megakaryocyte, and erythroid CSFs. Without a source of CSF, the cells died within 48 hr, but with CSF, cells entered an exponential growth phase within 18 hr with a doubling time of about 12 hr. Althogh growth was best with PWM‐SCM, this appeared to be due to increased numbers of surviving cells during the early period of culture and a decreased lag phase rather than a shortening of the doubling time. With PWM‐SCM, the absolute increase in cell number was 40‐fold. Over the culture period of seven days, the blast cells also differentiated morphologically to end cells, although differentiation appeared to be highly asynchronous. With MLCM and PMUE, only cells in the granulocyte‐macrophage series were seen, but with PWM‐SCM, erythroid cells were also observed. PMUE neither stimulated nor allowed the survival for longer than two days of granulocyte or erythroid committed progenitors (CFC) but, in contrast, stimulated a marked increase in macrophage CFC, which were uniquely responsive to PMUE stimulation. The proliferation of these progenitors appeared to be actively inhibited by PWM‐SCM. Despite the inability of MLCM to stimulate mature erythroid cell production, it was able to stimulate the early proliferation of erythroid CFC as well as granulocyte‐macrophage CFC. PWM‐SCM was able to stimulate the early and late proliferation of all types of CFC.