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Commitment and proliferation kinetics of human lymphocytes stimulated in vitro: Effects of colchicine on mitogen response
Author(s) -
Hall David J.,
O'leary James J.,
Rosenberg Andreas
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041120202
Subject(s) - colchicine , concanavalin a , population , biology , cell , in vitro , lymphocyte , peripheral blood , cell cycle , chemistry , microbiology and biotechnology , lag time , andrology , endocrinology , immunology , biochemistry , medicine , genetics , environmental health , biological system
Abstract We have examined the effects of colchicine on concanavalin A (Con A)‐ and phytohemagglutinin (PHA)‐stimulated human peripheral blood lymphocytes and from the time course of proliferation have extracted the relative size of the responding cell population, the rate of entry of this population into S‐phase, and the length of the lag period. Additions of colchicine at any time did not appear to influence the size of the responding population nor did it greatly affect the duration of the lag period. Only the rate at which the cell population enters initial S‐phase is a function of the time of previous exposure to colchicine. Colchicine does not appear to inhibit the commitment of stimulated lymphocytes to enter the cell cycle. Rather, it merely serves to decrease the biochemical processes responsible for fixing a maximal rate of entry into S‐phase.