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Ribosomal proteins are synthesized preferentially in cells commencing growth
Author(s) -
Tushinski Robert J.,
Warner Jonathan R.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041120119
Subject(s) - ribosomal protein , ribosomal rna , ribosome , protein biosynthesis , 5s ribosomal rna , biology , rna , 50s , 5.8s ribosomal rna , microbiology and biotechnology , biochemistry , chemistry , gene
Abstract Mouse 3T3 cells, in stationary phase because of serum deprivation, have only half the ribosome content of growing cells. Furthermore, the proportion of protein synthesis devoted to ribosomal proteins is only half that in growing cells. On addition of serum the synthesis of each ribosomal protein increases threefold, demonstrating the coordination of the synthesis of the ribosomal proteins. Half that increase is due to a general increase in total protein synthesis; half is due to a differential increase in ribosomal protein synthesis. The latter is abolished by a concentration of actinomycin D which blocks only ribosomal RNA transcription. The results are discussed with reference to a general hypothesis of growth regulation proposed by Stanners et al. (1979).