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Contact‐stimulated proliferation of cultured mouse epidermal cells by 3T3 feeder layers: Inhibition of proliferation by 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA)
Author(s) -
Miller Don R.,
Hamby Kathy M.,
Slaga Thomas J.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041120112
Subject(s) - clone (java method) , cell culture , epidermal growth factor , 12 o tetradecanoylphorbol 13 acetate , in vitro , microbiology and biotechnology , 3t3 cells , epidermis (zoology) , cell–cell interaction , biology , cell growth , chemistry , anatomy , transfection , biochemistry , signal transduction , dna , genetics , phorbol ester , protein kinase c
Mouse epidermal cells can be subcultured at 31°C onto an irradiated BALB/c 3T3 clone A31 feeder layer. A31 cells (supposedly derived from embryonic fibroblasts) were found to be specifically required for the optimal production of keratinizing epidermal colonies in secondary culture. This effect was not transmitted through the medium nor by the culture surface, since A31 cells plated on one end of a flask did not stimulate epidermal cell proliferation at the other end, even if the other end had previously held A31 cells. Epidermal cell contact with metabolizing A31 cells was probably necessary for the effect; fixed or freeze‐thawed A31 cells were ineffective. The tumor promoter 12‐O‐tetradecanoylphorbol‐13‐acetate, recently shown to interfere with contact‐mediated transfer of label (metabolic cooperation) between Swiss 3T3 cells and cells of an established epidermal line in vitro, also blocked epidermal colony formation. The A31‐epidermal cell interaction is apparently not a typical mesenchymal‐epithelial interaction, since the basement membrane would prevent this contact in intact skin.