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Immunological characterization of metallothioneins in mouse LMTK cells and in a variant resistant to cadmium
Author(s) -
Maiti Indu,
Mbikay Majambu,
Marengo Claude,
Thirion JeanPaul
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041120107
Subject(s) - metallothionein , ouchterlony double immunodiffusion , cadmium , radioimmunoassay , cell culture , biology , microbiology and biotechnology , fibroblast , gene , chemistry , biochemistry , antigen , immunology , genetics , antiserum , organic chemistry
A cadmium‐resistant variant isolated from mouse fibroblast LMTK cells can grow in the presence of 40 μM Cd 2+ . This variant retained its properties in the absence of selecting agent. Induction of metallothionein was measured in cell extracts by radioimmunoassay. The maximum amount of metallothioneins in the cells was reached after 36 hours. The cadmium resistant variant produced two times more metallothionein than the wild‐type when exposed to 10–20 μM Cd 2+ . By Ouchterlony double diffusion, the metallothioneins from cultured cells formed a line of parital identity with the mouse liver serotype and a line of complete identity with one of the two mouse kidney serotypes. These observations raise the possibility of a tissue‐specific expression of metallothionein genes.