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Perturbation of the switch‐on of transcriptase activity in intermediate subviral particles from reovirus
Author(s) -
Borsa J.,
Sargent M. D.,
Ewing D. D.,
Einspenner M.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041120104
Subject(s) - reverse transcriptase , microbiology and biotechnology , chemistry , biology , physics , biophysics , gene , virology , biochemistry , rna
Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch‐on of transcriptase function. In such particles the endogenous transcriptase is present in a switched‐off form, one step removed from the switched‐on state. Switch‐on of transcriptase function is an active process in this system and can be triggered by K + ions. A variety of agents which affect gene expression in cells were tested for an effect on switch‐on in ISVP. Marked effects on switch‐on in ISVP were observed with a diverse group of test agents, including DMSO and other solvents, BUdR, TdR, caffeine, theophylline, and temperature. The correlation in response between ISVP and cells suggests that the ISVP system may be useful as a model for studying the biochemical mechanisms underlying the perturbative effects of such agents on gene expression in cells.