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Inhibition of the serum‐dependent, amiloride‐sensitive sodium transport pathway in human fibroblasts by extracellular divalent cations
Author(s) -
Villereal Mitchel L.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041110207
Subject(s) - amiloride , ethylenediaminetetraacetic acid , chemistry , divalent , mole , sodium , extracellular , fetal bovine serum , stimulation , biochemistry , chelation , biophysics , in vitro , endocrinology , inorganic chemistry , biology , organic chemistry
Sodium influx in serum‐deprived human fibroblasts in a nominally Ca‐free, Mg‐free medium is significantly higher (17.8 ± 1.9 μmole/g prot/min) than that measured in a medium containing 1.8 mM Ca and 1 mM Mg (10.9 ± 0.7 μmole/g prot/min), and is stimulated dramatically (44.1 ± 6.1 μmole/g prot/min) by the addition of 10% fetal bovine serum (FBS), suggesting that an enhanced influx of Ca ions is not a necessary condition for serum activation of the amiloride‐sensitive Na influx pathway. The addition of 2 mM ethylenediaminetetraacetic acid (EDTA) to serum‐deprived cells in a low Ca, low Mg medium also results in a dramatic stimulation of Na influx (40.4 ± 3.7 μmole/g prot/min), while the addition of EDTA to cells assayed in a low Ca, low Mg medium in the presence of FBS has no significant effect on Na influx (45.3 ± 4.1 μmole/g prot/min). Thus, the stimulatory effects of FBS and EDTA are not additive. Kinetic analysis in the presence of varying amiloride concentrations indicate that the EDTA‐stimulated Na influx occurs via the amiloride‐sensitive Na pathway. The activation of Na influx in cells rinsed free of Ca and Mg Can be readily reversed by the addition of Ca or Mg to the assay medium. The Ca concentration required to give 50% inhibition of Na influx is 52 ± 7.6 μM (n = 3) for cells assayed in serum‐free medium and 272 ± 29 μM (n = 3) for cells assayed in the presence of 10% FBS. At physiological Ca concentrations (1.8 mM) the Na influx is maximally inhibited by Ca both in the presence and absence of serum. Since Na influx in 1.8 mM Ca medium is 2.5‐fold higher in the presence of serum than in its absence, these data suggest that the serum‐induced change in the K, for Ca modulation of the amiloride‐sensitive Na transport pathway is not sufficient to explain the serum stimulation of Na influx in human fibroblats.