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Effects of pyruvate on the growth of normal and transformed hamster embryo fibroblasts
Author(s) -
Sens Donald A.,
Hochstadt Bruce,
Amos Harold
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041100319
Subject(s) - embryo , hamster , glutamine , doubling time , biology , chinese hamster , cell culture , biochemistry , chemistry , cell , microbiology and biotechnology , in vitro , amino acid , genetics
The growth of NIL and NILpy hamster embryo fibroblasts was determined in the presence and absence of pyruvate as a component of the growth medium. It was demonstrated that NIL cells respond to the presence of pyruvate by decreasing cell doubling time, glucose utilization, glutamine utilization, and increasing lactate production with the effects being more pronounced at low inoculum densities. Polyoma‐virus transformed NIL cells (NILpy) demonstrate none of the above effects upon pyruvate addition regardless of initial cell inoculum density.