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Comparison of the effects of insulin and H 2 O 2 on adipocyte glucose transport
Author(s) -
Ciaraldi Theodore P.,
Olefsky Jerrold M.
Publication year - 1982
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1041100318
Subject(s) - insulin , trypsinization , spermine , medicine , endocrinology , chemistry , stimulation , glucose transporter , adipocyte , biochemistry , biology , adipose tissue , trypsin , enzyme
The abilities of insulin and the insulin mimickers spermine and H 2 O 2 to stimulate 3‐O‐methyl glucose transport in isolated rat ft cells were stuided in an attempt to determine possible common mechanisms of action. All three agents caused a seven‐ to 12‐fold stimulation of initial rates of glucose transport with insulin being the most effective agent. Insulin and spermine displayed similar time courses for the onset of their stimulation of transport; an initial lag before any effect was seen and then a gradual rise until the full effect was reached. The time course of H 2 O 2 activation of glucose transport was different since stimulation was seen at the earliest time point tested and then gradually rose to the maximal effect. Trypsinization of cells removed insulin receptors and rendered the cells insensitive to insulin but not to spermine or H 2 O 2 . However, trypsinization did alter the time course of H 2 O 2 action, causing an initial lag phase to appear and a general slowing of the activation kinetics. Pretreatment of cells with 2,4‐dinitrophenol to lower ATP levels prevented the stimulatory effects of insulin and the mimickers. All three of the agents revealed a similar temperature dependency for stimulated glucose transport, resulting in linear Arrhenius plots with activation energies of 10.2–11.4 kcal/mole. These results show that (1) H 2 O 2 does not act directly on the glucose transport system of rat adipocytes and (2) insulin and H 2 O most likely act through a common energy‐dependent biochemical pathway to stimulate glucose transport, but H 2 O 2 enters the stimulus‐response sequence distal to the initial steps in insulin action.